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wren2land (#250) 5 days ago
Plates, and the numbers of hatched eggs quantified 36 h later.Ubiquitin Ligase AssayLigase assays were performed essentially as described [24] for 45 minutes at 37uC with the following components: 50 mM TrisHCl pH 7.5, 5 mM MgCl2, 2 mM NaF, 0.6 mM DTT, 2 mM ATP, 10 nM Okadaic Acid, 40 ng rabbit Ube1 (Boston Biochem), 300 ng UbcH5, 12 mg bovine Ub (Sigma), and 250 ng GST-Roc. Samples were run by SD